Bozeman video ap biology labs prelab questions dissolved oxygen. While i was recently going over the dissolved oxygen data from lab 12, i realized that the nomograph in edition d page 140 of the ap lab manual is very poor and inaccurate. In this classic water quality testing lab, the relationship between the amount of dissolved oxygen in water and water quality are explored and quantified using the winkler method. Remove the do probe from the storage bottle and carefully insert it into the culture. Ap biology lab 12 allowed students to investigate and evaluate the physical. From your graph, what is the effect of temp on the amt of oxygen that water at differnet temps can hold. Jun 09, 2017 ap biology dissolved oxygen and primary productivity lab anthony garcia and frank cui. Net primary productivity is at its height at 0 meters depth in pond. To determine whether the temperature of the water impacts the rate in which oxygen is dissolved in water. Primary productivity lab modified by sean mcglynn 2012 cibt alumni workshop animals ecology high school inquiryscientific method physical sciences plants.
Dissolved oxygen and aquatic primary productivity lab. Analyze the effect of varying amounts of light on primary productivity. Investigate factors that may affect primary productivity in a natural system. Propose an alternate experimental design that would have allowed us to assess primary productivity. Dissolved oxygen and primary aquatic productivity laboratory 12 introduction dissolved oxygen levels are an extremely important factor in determining the quality of an aquatic environment. Dissolved oxygen and primary aquatic productivity laboratory 12. Relate the importance of dissolved oxygen to the function of aquatic ecosystems. Videos anatomy and physiology ap biology ap chemistry. Dissolved oxygen and aquatic primary productivity kit. Great as a field activity to study biochemical oxygen demand bod, respiration rate and primary productivity. I now have a better understanding of why ecosystems create and or need dissolved oxygen and the whole process of primary productivity. A biology student inadvertently removed all the screens and labels from the watersampling bottles before he measured the amount of dissolved oxygen. Describe a method to determine net and gross primary productivity in a freshwater pond over a 24hour period. The graphs shows that the lower the water, the less primary production is occurs.
What method of determining the amount of dissolved oxygen in a water source is used in this lab. Students will use lamotte test kits to determine the concentration of dissolved oxygen do in various water samples. Ap biology 2008 scoring guidelines form b question 1 1. So what the algae really produced in the bottle was a total of 15mg dissolved oxygen, it just lost 5mg to respiration. The advanced placement biology laboratory manual recommends that each.
In exercise 12b you will measure and analyze the primary productivity of natural waters or lab cultures using screens to simulate the. Measure the amount of dissolved oxygen in water samples at different temperatures. Investigation dissolved oxygen and aquatic primary productivity. They span the globe in their topics and their techniques. In general, how would you expect turbidity to affect the productivity of a lake. From this lab i have learned that the amount of dissolved oxygen created varies on the ecosystem itself and the factors in the ecosystems that effect the dissolved oxygen level. Explain the relationship of dissolved oxygen to primary productivity. When he tested the unidentified bottles, he obtained the results shown below. Ap biology dissolved oxygen and primary productivity lab anthony garcia and frank cui. Since 1989, there have been 12 laboratorieslovingly nicknamed the dirty dozenthat are suggested curricula for ap biology classes.
Ap biology lab 12 dissolved oxygen and aquatic primary productivity overview 1. Dissolved oxygen and primary productivity 6 for evaluation purposes only 39. Raymond chen, nikhil jithoo, asish katta, noah schneider. The advanced placement ap program is a registered trademark of the college. In an aquatic environment, oxygen must be dissolved in order to be available for use by organisms.
These suggestions are provided to enhance the students overall lab experience as well as their conceptual understanding. Primary productivity lab modified by sean mcglynn cornell. Simulate and record primary productivity using chlorella. Gross productivity is amount of oxygen use for photosynthesis. In exercise 12b you will measure and analyze the primary productivity of natural waters or lab cultures. Click on the pdf file below, read and answer the questions see handout from classanswer in your composition notebook. Dissolved oxygen and aquatic primary productivity introduction in an aquatic environment, oxygen must be dissolved in order to be available for use by organisms. In part b, you will you will measure and analyze the primary productivity of natural waters or. Dissolved oxygen and aquatic primary productivity by. Instructions introduction key concepts dissolved oxygen. To be useful to aquatic organisms, oxygen must be in the form of molecular oxygen, o 2. This lab is a fitting climax to the series of 12 aprecommended biology labs. Think about the components of a good experiment identify variables, etc. I compared it to a chart of 100 percent oxygen solubility at different temperatures, and in all cases for the chart values at 100 percent, the nomograph reads.
Dissolved oxygen and aquatic primary productivity measure amount of o2 consumed dissolved oxygen do after exposure to light or darkness by titration difference between initial and light net productivity photosynthesis increases do. Dissolved oxygen and aquatic primary productivity lab sammi. Kindle file format ap bio lab 12 dissolved oxygen answers. In the atmosphere, oxygen comprises over 20% of the available gases. Containers other than beakers can be used, but remember that the. In exercise 12a your students will measure and analyze the dissolved oxygen do concentration in water. An aerator was producing a constant stream of bubbles, so they should have had plenty of oxygen. The silver ring on the probe should be immersed in the culture, but the probe should not. As you may know, people have search hundreds times for their chosen novels like this ap bio lab 12 dissolved oxygen answers, but end up in harmful downloads.
Pearson, as an active contributor to the biology learning community, is pleased to provide free access to the classic edition of the biology place to all educators and their students. Diffusion and osmosis overview the information will assist teachers with aspects of lab 1 that are not necessarily addressed in the lab manual. In exercise 12a you will measure and analyze the dissolved oxygen do concentration in water samples at varying temperatures. Ap sample lab 12 dissolved oxygen biology junction. Always wash hands thoroughly with soap and water after handling reagents or bio. Clearly explain the difference between gross primary productivity gpp and net primary productivity npp 1b. Dissolved oxygen levels are used to monitor the health and productivity. Ap bio lab 12 dissolved oxygen answers ap bio lab 12 dissolved thank you for downloading ap bio lab 12 dissolved oxygen answers. Ap lab 12 prelab dissolved oxygen and aquatic primary. Ap biology lab 12 dissolved oxygen and aquatic primary. The initial oxygen reading for this water was 4 mg o 2 l. Ap biology lab manual for teachers supplement lab 1.
If the temperature of the water is increased, then there is no effect on the rate of dissolution of oxygen inside water iv. The new ap biology labs can be found online by clicking the link below. Andersen demonstrates the azidewinkler method of dissolved oxygen. In this exercise, you will measure and analyze the dissolved oxygen do concentration in water samples at varying temperatures. For example, 5 mgl of do in 20degree water may represent a 50. Dissolved oxygen is necessary for the metabolic processes of almost every organism. Using the following diagram describe the effect that. It applies knowledge gained in several of the other labs, especially lab 4 on photosynthesis and lab 5 on cellular respiration. Andersen demonstrates the azidewinkler method of dissolved oxygen analysis. So what the algae really produced in the bottle was a total of 15mg dissolved oxygen, it. In fact, there is a point in which net primary production is less than zero. If npp for an ecosystem is positive, what will happen to the biomass of that ecosystem over time. Terrestrial environments hold over 95% more oxygen than aquatic environments. To determine whether the temperature of the water impacts the rate in which oxygen is dissolved in water hypothesis.
The silver ring on the probe should be immersed in the culture, but the probe should not be touching the bottom of the beaker. Dissolved oxygen and primary productivity lab youtube. Dissolved oxygen levels are an extremely important factor in determining the. Biology with vernier 25 1 primary productivity ap bio lab 12b oxygen is vital to life. Why is it necessary to know the water temperature before beginning this lab. Define terms in your composition notebook see handout from class part 3. Apr 08, 2010 ap biology lab 12 dissolved oxygen and aquatic primary productivity. Ap biology lab 12 allows students to investigate and evaluate the physical and biological factors that affect the solubility of dissolved oxygen and subsequent effects on aquatic ecosystems of earth. Measurements of dissolved oxygen do are used to determine primary productivity in bodies of water. Use the winkler technique to measure dissolved oxygen levels. We cannot measure gross productivity directly because respiration, which uses up oxygen and organic compounds, is always occurring simultaneously with. Using the following diagram, describe the effect that each of the factors has on availability of oxygen and.
The concentration of dissolved oxygen in an aquatic environment is an important indicator of the environments water quality. Investigation dissolved oxygen and aquatic primary. At depth of 25 meters in water, net primary production has no increase or decrease. Net productivity is amount of oxygen used in respiration and released in photosynthesis.
Explain the difference between gross and net primary productivity. Dissolved oxygen and aquatic primary productivity safety preparation and presentation. Dissolved oxygen and primary productivity ap central place one beaker in an ice bath or refrigerator, place one beaker at room temperature, and place one beaker in a 30c water bath or. These laboratory materials have been prepared by edvotek, inc. Dissolved oxygenclassic lab kit for ap biology, 8 groups. Measure the amount of dissolved oxygen in water samples at different temperatures 2. If cool water is heated carefully without driving out the oxygen, the percent saturation will go up. In this investigation, you will measure and analyze the dissolved oxygen do concentration in water samples at varying temperatures. Energy dynamics primary productivity matters to ecosystems because it allows us to determine how much energy is available for the higher trophic levels.
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